Toxic effect of Lantana camara on Aedes aegypti larvae: A scanning electron microscopic study

Research Article

Toxic effect of Lantana camara on Aedes aegypti larvae:

 A scanning electron microscopic study

Author: Dr. Anju R Unnithan*, Dr. Geetha Unnikrishnan**

*Department of Biotechnology, Birla College of Arts Science and Commerce, Kalyan, India.

**Department of Zoology, Birla College of Arts Science and Commerce, Kalyan, India.

Corresponding Author: Dr. Anju R Unnithan (anjurunnithan@gmail.com)

Abstract:

Background & Objective: In the present study the morphological changes induced by Lantana camara petroleum ether extract on Aedes aegypti third instar larvae is analyzed to validate its toxic effect leading to the death of the larvae.

Method: LC50 for 24hr exposure time of instar larvae of Aedes aegypti against Lantana camara petroleum ether extract leaf extract was calculated. SEM analysis was performed by scanning control and extract treated larvae in SEM ‘ CARL ZEISS EVO 40’ at 20 kV.

Results: LC50 value of Lantana camara petroleum ether leaf extract for 24hr exposure time was estimated as 48.851ppm. SEM analysis of post-treatment larvae showed distortions in the head and thorax region. Mouth region, especially the mouth brushes was disrupted and constrictions were observed on the head. Abdomen (Midgut region) was entirely malformed and the anal papillae were utterly disrupted Siphon was not affected showing normal spiracular valves with little changes on the surface of tracheal trunk and muscle fibers.

Conclusion: Present study provides a scientific rationale to highlight the importance of Lantana camara petroleum ether leaf extract as a source of larvicidal agents against Aedes aegypti.

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